Coding

Part:BBa_M36581

Designed by: Aris Kare, Ricky Cordova, Eda Benites   Group: Stanford BIOE44 - S11   (2015-10-24)

ctfA Gene Sequence

This part is the ctfA gene, or butyrate-acetoacetate COA-transferase subunit A, that is used as part of a metabolic pathway in Clostridium acetobutylicum ATCC 824 bacteria to produce acetone. We are aiming to use this gene, along with several others, to eventually produce acetone and CO2 in E.coli bacteria. This part, along with ctfB, produces acetoacetate, acetyl-CoA, and butyryl-CoA, which are the penultimate products of the C. acetobutylicum acetone pathway before the final product, acetone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/actfA Gene Sequence