Part:BBa_M36567

Actuator to reduce Hg(II) to elemental Hg.

Encodes transport and reduction of toxic Hg (II) ion to volatile, low-toxicity elemental Hg. This actuator is based off of naturally occurring operon genes in e.coli with evolved Hg(II) resistance and reduction. The self regulatory features of the natural system have been removed and only the necessary components remain in the actuator. merT transports the Hg(II) ions across the cell membrane and into the cytoplasm of the cell. merP aids in the transportation and begins the early stages of the ionic reduction. merA codes for Mercuric Reductase, the enzyme responsible for the reduction to the ionic form of Hg. The actuator accepts a PoPS signal and, in the presence of Hg(II) ions, will reduce the ions to the elemental form. This actuator can be attached to sensors like Rhamex 67K, induced with 1000umol, rhamnose.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1741
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1741
  Illegal NotI site found at 436
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1741
  Illegal BamHI site found at 1629
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1741
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1741
  Illegal NgoMIV site found at 652
  Illegal NgoMIV site found at 2028
  Illegal AgeI site found at 727
  Illegal AgeI site found at 982
  Illegal AgeI site found at 1423
  Illegal AgeI site found at 1840
  Illegal AgeI site found at 1993
  
• 1000
  COMPATIBLE WITH RFC[1000]