Part:BBa_M36557

VItamin D2 Actuator

This device converts ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. In the presence of UV light ergosterol is converted to Vitamin D2. The coding region[BBa_M36655] produces C-24(28) sterol reductase an enzyme that catalyzes the final step in Vitamin D2 synthesis pathway by converting ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol.

Design Notes

The encoded enzyme sequence [BBa_M36655] has to be codon optimized to the actuators host.While [BBa_M36655] is already optimized for expresion in Synechococcus elongatus (PCC 7942), the unchanged enzyme coding sequence can be obtained from the NCBI database Gene ID :2541740 , for optimization for different host organism. However, the suggested host organism is S. elongatus as it can withstand UV light radiations( necessary to convert ergosterol to VItamin D2) as it naturally carries out photosynthesis. It is unfeasible to deploy the actuator in E.Coli since Vitamin D is toxic to E.Coli. In order to work with E. Coli, the strain of E.Coli has to be Vitamin D resistant.

Sources and References

Tomoko Ikwaki,Haruyuki lefuji,Yoshikazu Hiraga,Akira Hosomi, Tomotake Morita, Yuko GigaHamam Kaoru Takegawa.”Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe”.7 December 2007. A direct link to the paper is available below http://mic.sgmjournals.org/content/154/3/830.full

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1028
  Illegal AgeI site found at 1289
  
• 1000
  COMPATIBLE WITH RFC[1000]