Composite

Part:BBa_M36555

Designed by: Christian Castaneda, Thai Nguyen, Andrew Rodriguez   Group: Stanford BIOE44 - S11   (2012-12-06)


Ferredoxin-producing gene with iron sensor

The ferredoxin, which is an iron-sulfur protein, is constitutively produced at high levels. Ferredoxin sequesters iron, so an iron sensor in the form of an iron-inducible promoter is put downstream of the ferredoxin gene. It may be possible to calculate the amount of ferredoxin produced by controlling the dissolved iron concentration and measuring the output of a reporter put downstream of the iron sensor. Comparing this result to a positive control of the reporter output of cultures with only the iron sensor would enable the calculation (part BBa_I716002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 185
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 103
    Illegal SapI.rc site found at 523


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Categories
Parameters
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