Composite

Part:BBa_M36552

Designed by: Aprotim Cory Bhowmik, Cali Nguyen, Zoe Lee-Chiong   Group: Stanford BIOE44 - S11   (2015-10-22)


OYE1 Composite Sequence

Consists of multiple parts to produce the Old Yellow Enzyme 1 (OYE1) to reduce alpha-beta unsaturated C=C double bonds. Specifically consists of a rhamnose-inducible promoter (P_rha_BAD), a strong RBS, the OYE1 coding sequence, a His_* tag, and a termination sequence from T7.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
    Illegal PstI site found at 677
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
    Illegal PstI site found at 677
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
    Illegal PstI site found at 677
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 956
    Illegal PstI site found at 677
    Illegal NgoMIV site found at 218
    Illegal NgoMIV site found at 266
    Illegal NgoMIV site found at 272
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None