Coding

Part:BBa_M36551

Designed by: Aprotim Cory Bhowmik, Cali Nguyen, Zoe Lee-Chiong   Group: Stanford BIOE44 - S11   (2015-10-22)


OYE6 Protein Sequence

Codes for the Old Yellow Enzyme 6 (OYE6) from Achromobacter sp. OYE6 is part of the Old Yellow Enzyme (OYE) family, all of which are thought to function in reducing alpha-beta unsaturated C=C double bonds. In our experiment, we are using OYE6 to reduce citral to citronellal (by reducing an alpha-beta unsaturated C=C double bond in citral).

OYE6 can be used by simply coding for the enzyme in a DNA sequence. As a result, the enzyme should be produced and can be used to reduce certain compounds that need to be introduced separately in vitro or in vivo.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1072
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1072
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1072
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1072
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1072
    Illegal NgoMIV site found at 187
    Illegal NgoMIV site found at 420
    Illegal NgoMIV site found at 1038
    Illegal AgeI site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None