Coding
N4 gp8

Part:BBa_M36548

Designed by: Rashmi Sharma, Bryce Bajar, Kendrick Wang   Group: Stanford BIOE44 - S11   (2011-12-07)

DNA Polymerase Inhibitor (via N4 coliphage gp8)

This N4-derived gene (gp8) produces a small protein which binds to the delta-subunit of DNA Polymerase III in E. Coli to stop DNA replication.

Usage and Biology

Successfully inhibits DNA replication in K-12 strain of E. coli. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/dna
//proteindomain/binding
Parameters
chassisE. coli
protein_outN4 gp8