Plasmid

Part:BBa_M36534

Designed by: Inderpreet Kaur Hayer   Group: Stanford BIOE44 - S11   (2015-10-24)

Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes

This part was made in order to reduce arsenate to arsenite and enhance arsenite resistance in S. cerevisae.

Construct uses DNA 2.0 plasmid backbone (pD1207) with GAL1 inducible (galactose-inducible/glucose-repressible) promoter, high copy number ORI, and kanamycin and histidine as selectable markers in E. coli and yeast, respectively. A self-cleaving 2A peptide sequence was inserted between ACR2 and ACR1 genes to allow for both genes to be transcribed.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 873
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None