Part:BBa_M36458
PoPS -> Cell Lysis
This actuator, upon receiving a Polymerase per Second (PoPS) signal, creates 3 proteins that induce cell lysis in E. Coli. Each protein is paired with a Ribosome Binding Site already included in the sequence. This part has been proven to have consistent success in cell lysis of E. Coli. The three proteins transcribed from the sequence are a Holin (lysis the cell by inserting itself in the plasma membrane and causing cell lysis after reaching a critical mass), an Endolysin (destabilizes the cell wall), and an Rz Protein (helps the holin enter the plasma membrane). The sequence is void of all repeats and palindromes greater than or equal to 10 bp thus allowing it to be synthesized with relative ease. To use this product one would simply have to pair this part with a sensor that would then send a PoPS signal and cause the cell to lyse. This actuator has been proven to cause lysis even at very low levels of activation. Despite this, it has also been shown to not cause lysis unless activated.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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