DNA

Part:BBa_M36430

Designed by: Anna Jaffe   Group: Stanford BIOE44 - S11   (2014-10-23)

Bxb1 attP - reverse

The Bxb1 integrase catalyses recombination of Bxb1-attB with Bxb1-attP. If the 2 sites are in the same molecule, recombination outcome can be: a) excision of the sequence in between (att sites in parallel orientation), or b) sequence inversion (att sites in antiparallel orientation). This is the Bxb1-attP site in the reverse orientation, to result in the "flipping" rather than the "exicision" outcome when used behind an attB site in the same orientation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 22


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Categories
Parameters
None