Translational_Unit

Part:BBa_M36428

Designed by: Anna Jaffe   Group: Stanford BIOE44 - S11   (2014-10-23)

RBS, spacer, Bxb1 integrase

This part encodes the necessary translational components for the expression of the Bxb1 integrase (RBS, 6-bp spacer, integrase sequence) and can be placed behind the promoter of the user's choice for differing expression levels.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1447
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1447
    Illegal AgeI site found at 1501
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None