RBS

Part:BBa_M36405

Designed by: Justo A. Caballero, Shamik Mascharak and Sibel Sayiner   Group: Stanford BIOE44 - S11   (2012-12-05)


Bicistronic RBS (medium) pFAB1646

This is a bicistronic RBS, which consists of a constitutive RBS, and a leader sequence that includes the second RBS. It uses bicistronic junction architecture to tightly control translation. It contains two ribosome binding sites: one controls the gene of interest, and the another synthesizes a short leader protein. The latter bicistronic's translation prevents the former RBS from forming a secondary structure with the gene of interest. This ensures a more consistent expression level of your gene of interest.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None