Part:BBa_M36362
DbjA Haloalkane Dehalogenase
This part encodes an enzyme with dehalogenase activity for haloalkane substrates. DbjA catalyzes the cleavage of carbon-halogen bonds to form a primary alcohol and a halide. DbjA generally has higher overall activity than other haloalkane dehalogenases. Unlike most other known haloalkane dehalogenases, DbjA has very high activity on 2-bromo-1-chloropropane and chlorocyclopentane, and it has significant activity towards 1,2-dichloropropane.
ATG and TAA stop codons are included, so this part can just be flanked by a 5' UTR translation initiator and a 3' transcription terminator for use.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 379
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 379
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 379
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 379
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 379
Illegal NgoMIV site found at 895
Illegal AgeI site found at 624 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 685
References
Koudelakova, T., Chovancova, E., Brezovsky, J., Monincova, M. et al., Substrate specificity of haloalkane dehalogenases. Biochem. J. 2011, 435, 345–354.
Sato, Y., Monincova, M., Chaloupkova, R., Prokop, Z. et al., Two rhizobial strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, encode haloalkane dehalogenases with novel structures and substrate specificities. Appl. Environ. Microbiol. 2005, 71, 4372–4379.
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