Part:BBa_M36307

FseI restriction endonuclease actuator construct

This composite part includes a strong bicistronic RBS, the FseI protein coding gene, and a pT-T7 terminator.

Usage and Biology

The coding region produces the restriction enzyme FseI. As FseI is produced, it cleaves DNA at recognition sites using exonuclease activity. This can be utilized for experiments by surrounding a gene of interest with FseI cut sites. Under the control of a promoter of choice, FseI can be expressed and cut out either the promoter or entire gene in the gene of interest. This way, one can control temporally the knock out of a gene of interest.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 532
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 595
  Illegal NheI site found at 761
  Illegal PstI site found at 532
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 431
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 532
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 532
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 94