DNA

Part:BBa_M36301

Designed by: Darren Jindal, Raman Nelakanti, Dennis Te   Group: Stanford BIOE44 - S11   (2011-12-08)

Dual FseI restriction sites bounded by spacers

The part is a DNA sequence that acts a restiction enzyme cutting site for FseI. This guards against methylation of the DNA for reliable cutting.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None