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Part:BBa_M36294

Designed by: Dominique Dabija   Group: Stanford BIOE44 - S11   (2011-12-06)


Spider Silk Actuator

This is the full sequence for the spider silk actuator. It includes the bicistonic sequence, FOS, a linker, the silk hexamer, another linker, JUN, a stop codon, and a transcription terminator. It is triggered by PoPS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 306
    Illegal PstI site found at 348
    Illegal PstI site found at 707
    Illegal PstI site found at 1595
    Illegal PstI site found at 1992
    Illegal PstI site found at 2034
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 306
    Illegal PstI site found at 348
    Illegal PstI site found at 707
    Illegal PstI site found at 1595
    Illegal PstI site found at 1992
    Illegal PstI site found at 2034
    Illegal NotI site found at 499
    Illegal NotI site found at 1387
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 306
    Illegal PstI site found at 348
    Illegal PstI site found at 707
    Illegal PstI site found at 1595
    Illegal PstI site found at 1992
    Illegal PstI site found at 2034
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 306
    Illegal PstI site found at 348
    Illegal PstI site found at 707
    Illegal PstI site found at 1595
    Illegal PstI site found at 1992
    Illegal PstI site found at 2034
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 860
    Illegal BsaI site found at 1748


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