Device

Part:BBa_M36279

Designed by: Victoria Robles, Kyung Ri Park, Ken Xiong   Group: Stanford BIOE44 - S11   (2011-12-09)


Copper Sensor --> PoPS

This device sends PoPs signals in response to the presence of copper. It consists of a strong constitutive promoter, a bicistronic RBS, copZ, another bicistronic RBS, copY, a transcription terminator, and cop promoter. The copY protein represses the cop promoter, but if copper attaches to copY, copY will release from the promoter, and PoPs will be produced. The copZ protein acts as a copper chaperone, transporting copper to copY attached to the cop promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 268
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 287


[edit]
Categories
Parameters
None