Coding

Part:BBa_M36264

Designed by: Diana Gong   Group: Stanford BIOE44 - S11   (2014-10-23)


LasR optimized for E. coli

Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasR binds to N-(3-oxododecanoyl)-homoserine lactone, which is produced by LasI, to form a complex that regulates quorum sensing-related genes.

This part begins with a start codon and ends with the stop codon for the LasR gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 67
    Illegal PstI site found at 475
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 298
    Illegal NheI site found at 79
    Illegal PstI site found at 67
    Illegal PstI site found at 475
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 67
    Illegal PstI site found at 475
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 67
    Illegal PstI site found at 475
    Illegal AgeI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None