Coding

Part:BBa_M36263

Designed by: Diana Gong   Group: Stanford BIOE44 - S11   (2014-10-23)


LasI optimized for E. coli

Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasI produces N-(3-oxododecanoyl)-homoserine lactone, and LasR detects it.

This part begins with a start codon and ends with the stop codon for the LasI gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 397
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 397
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 397
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 397
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None