Part:BBa_M36239
Epinephrine sensor construct
This part is designed to sense epinephrine, and generate a PoPS signal in response. The QseB and QseC genes are connected to a constitutive promoter, and so will be constantly expressing the QseB and QseC proteins, which will creates the adrenergic receptors (QseC) on the surface of the cell as well as the signaling molecules activated when the receptor is activated (QseB). When epinephrine is detected by QseC, it autophosphorylates, which in turn will phosphorylate QseB. This, in turn, will bind to the flhDC promoter, activating it. This will cause a PoPS signal to be produced, which can be used with an actuator. It is important to note that QseC may be activated not only by epinephrine, but also norepinephrine and Auto-Inducer 3 (AI3).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1949
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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