Composite

Part:BBa_M36224

Designed by: Joel Dominguez, Ashwin Raghunathan, Denis Willett   Group: Stanford BIOE44 - S11   (2012-12-04)

Red Luciferase for E. coli

A red luciferase (peak luminescence at 622nm) gene originally from Phrixothrix hirtus and optimized for use in E. coli. Requires D-luciferin substrate for luminescence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal PstI site found at 152
    Illegal PstI site found at 1085
    Illegal PstI site found at 1454
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal PstI site found at 152
    Illegal PstI site found at 1085
    Illegal PstI site found at 1454
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal BglII site found at 240
    Illegal BglII site found at 1245
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal PstI site found at 152
    Illegal PstI site found at 1085
    Illegal PstI site found at 1454
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 629
    Illegal EcoRI site found at 887
    Illegal EcoRI site found at 1502
    Illegal PstI site found at 152
    Illegal PstI site found at 1085
    Illegal PstI site found at 1454
    Illegal NgoMIV site found at 1429
    Illegal AgeI site found at 680
    Illegal AgeI site found at 1211
    Illegal AgeI site found at 1337
    Illegal AgeI site found at 1658
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None