Composite

Part:BBa_M36222

Designed by: Designed by Cyonna Holmes, Martha Roman, Terry Xu   Group: Stanford BIOE44 - S11   (2011-12-06)

ArsAction: Arsenite Removal Device (input: PoPs Signal, output: removal device)

The arsenic removal device is designed to prolong cell survival and decrease the rate of cell death due to arsenite toxicity. It confers low level resistance to arsenite. Our device does this by removing arsenite from the comprimsed cell using a two part removal method. The first coding sequence, arsA, is an ATPase that localizes to the membrane of the cell and confers energy to ArsB. ArsB uses this energy to form an ion efflux pump designed to drive arsenite out of the cell. This device is to be used as an actuator and it responds to the universal PoPs signal. The genes used are specific to the arsenic family and can only be used to create a pump that removes the ion form of arsenic (arsenite).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 319
    Illegal EcoRI site found at 709
    Illegal PstI site found at 1487
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 319
    Illegal EcoRI site found at 709
    Illegal PstI site found at 1487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 319
    Illegal EcoRI site found at 709
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 319
    Illegal EcoRI site found at 709
    Illegal PstI site found at 1487
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 319
    Illegal EcoRI site found at 709
    Illegal PstI site found at 1487
    Illegal NgoMIV site found at 1873
    Illegal AgeI site found at 472
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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