Composite
ADHAc

Part:BBa_M36203

Designed by: Kevin Aliado, Steven Lee, Simon Zheng   Group: Stanford BIOE44 - S11   (2011-12-06)


ADH Actuator; input is PoPS and output is ADH enzyme

This actuator receives an input of polymerases per second from a standard sensor and produces ADH1 (Alcohol Dehydrogenase 1) and ADH2 linked to a 6-histidine tag for any necessary for quantitative assays.

This actuator utilizes a bicistronic RBS to prevent binding of the RBS with any other parts of the coding sequence and to maintain consistency in the transcription rate.

This actuator consists of ADH1, which converts acetaldehyde to ethanol, and ADH2, which converts ethanol to acetaldehyde. ADH1 is activated by the presence of acetaldehyde, while ADH2 is activated by the presence of ethanol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1721
    Illegal PstI site found at 977
    Illegal PstI site found at 1076
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1721
    Illegal PstI site found at 977
    Illegal PstI site found at 1076
    Illegal NotI site found at 1209
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1721
    Illegal BglII site found at 952
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1721
    Illegal PstI site found at 977
    Illegal PstI site found at 1076
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1721
    Illegal PstI site found at 977
    Illegal PstI site found at 1076
    Illegal AgeI site found at 715
    Illegal AgeI site found at 749
    Illegal AgeI site found at 940
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 605
    Illegal SapI site found at 1953


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Categories
Parameters
None