Composite

Part:BBa_M36195

Designed by: Everett Frost   Group: Stanford BIOE44 - S11   (2012-06-03)


Bicistronic sequence containing ligninase and required enzyme manganese peroxidase

The part contains a ligninase coding sequence attached to a manganese sequence through the use of a custom designed linker sequence. All the source information can be found under their part descriptions: 1. https://parts.igem.org/Part:BBa_M36191 2. https://parts.igem.org/Part:BBa_M36192 3. https://parts.igem.org/Part:BBa_M36187

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 355
    Illegal PstI site found at 930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 355
    Illegal PstI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 909
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 355
    Illegal PstI site found at 930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 355
    Illegal PstI site found at 930
    Illegal NgoMIV site found at 477
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 334


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Categories
Parameters
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