Regulatory

Part:BBa_M36177

Designed by: Majed Magzoub, Guhan Venkataraman, Joe Carpenter   Group: Stanford BIOE44 - S11   (2015-10-23)

pH sensor

pH sensor, active within range 5.5-7.0. Our part is based on the acid inducible promoter region of a rcFB gene (producing rcFB protein) in Lactococcus lactis. Our assumptions are as follows: (1) generally Lactococcus lactis genes will work in E. coli, (2) all transcription factors specific to this rcFB gene are endogenous to E. coli, (3) there is no additional layer of feedback controlling the rcFB gene, (4) the rcFB gene is more responsive to lactic acid/lactate than carbohydrates (to which it also reacts), (5) base level of transcription is low in comparison to when gene is turned on.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 386
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None