Coding

Part:BBa_M36160

Designed by: Eric Kofman, Matt Kandath   Group: Stanford BIOE44 - S11   (2012-06-04)

T7 17.5 protein (holin)

Coding sequence for 17.5 protein in T7 phage. The 17.5 protein functions as a holin: in gram-negative bacteria, the holin degrades the inner peptidoglycan cell wall. For complete lysis to occur, an endolysin protein is also required (in T7 phage, this is the 3.5 protein).

This sequence should be preceded by a start codon (start codon is not included in this sequence). A stop codon, TGA, is included in this sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None