Part:BBa_M36158
E.coli Temperature Shock Sensor/Actuator
The structure of this part is the cold shock promoter (BBa_M36156), followed by a ribosome binding site (BBa_B0034), meffBlue (BBa_M361567), a transcriptional terminator (BBa_B1006), and finally a heat shock promoter (BBa_K338001). This temperature shock sensor/actuator produces Comet, a green fluorescent protein, or meffBlue, a blue chromoprotein, under heat shock or cold shock, respectively. MeffBlue, part BBa_B0034, is placed downstream of the cold shock promoter, part BBa_M36156, to induce the synthesis of this blue protein under cold conditions. Although not included in this DNA design, if placed carefully, this DNA design can be inserted in the DNA backbone of E.coli, that already contains Comet, upstream of which contains a ribosome binding site such that transcription of Comet can be activated by the heat shock promoter, part BBa_K338001.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 656
Illegal AgeI site found at 764 - 1000COMPATIBLE WITH RFC[1000]
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