Composite

Part:BBa_M36154

Designed by: Monica Chin   Group: Stanford BIOE44 - S11   (2014-10-23)


NifA sensor

This sensor functions by responding to nitrogen. The NifRLA promoter induces the transcription factor NifA. NifA regulates transcription of the rest of the nif gene. In this case, NifA activates the NifHDK promoter, which will interact with our reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 499
    Illegal PstI site found at 649
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 499
    Illegal PstI site found at 649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1539
    Illegal BglII site found at 2003
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 499
    Illegal PstI site found at 649
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 499
    Illegal PstI site found at 649
    Illegal NgoMIV site found at 921
    Illegal NgoMIV site found at 2044
    Illegal AgeI site found at 1801
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None