Regulatory

Part:BBa_M36145

Designed by: Jane Hae Soo Shin   Group: Stanford BIOE44 - S11   (2012-06-06)


Cold-inducible promoter

This promoter is up-regulated by lowering temperature (to 33 degree Celsius) and it was found in CHO cells. It has been discovered so far that the transcription factors for this promoter are NF-kB and SP1 which are endogenous to both CHO cells and HeLa cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 142
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 112
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 202


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Categories
Parameters
None