Regulatory

Part:BBa_M36138

Designed by: Lea Jabbour   Group: Stanford BIOE44 - S11   (2015-10-22)

Lac Operon modified CAP binding site

In order to create an E. coli lactose sensor without the interference of glucose, we are altering the CAP binding site to nullify the effect of cAMP--and therefore glucose--dependence, and make transcription only dependent on lactose presence. This promoter has a modified CAP binding site sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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