Regulatory
LacOp Del

Part:BBa_M36137

Designed by: Lea Jabbour, Kayla Clough   Group: Stanford BIOE44 - S11   (2015-10-22)

Lac Operon deleted CAP binding site

In order to create an E. coli lactose sensor without the interference of glucose, we are altering the CAP binding site to nullify the effect of cAMP--and therefore glucose--dependence, and make transcription only dependent on lactose presence. This promoter has a deleted CAP binding site sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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