RNA

Part:BBa_M36134

Designed by: Daniel McHugh   Group: Stanford BIOE44 - S11   (2012-06-01)


1D inducible RNA scaffold

Self-assembles into a one-dimensional RNA scaffold with binding domains for the MS2 bacteriophage coat protein. When presented with theophylline, the ribozyme should cleave the scaffold sequences, releasing the aptamers from the existing scaffold and preventing other scaffolds from forming.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 193
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 193
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 193
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 193
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None