RNA

Part:BBa_M36132

Designed by: Vir Choksi, Daniel McHugh   Group: Stanford BIOE44 - S11   (2012-06-01)


2D inducible RNA scaffold

Self-assembles into a two-dimensional RNA scaffold with binding domains for MS2 and PP7 proteins. When presented with theophylline, the ribozyme should cleave the scaffold sequences, releasing the aptamers from the existing scaffold and preventing other scaffolds from forming.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 71
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 71
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 71
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 71
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None