Device

Part:BBa_M36124

Designed by: Daniel Hart, Gustavo Garcia, Raymond Serrano   Group: Stanford BIOE44 - S11   (2015-11-05)


Glucose-6-Phosphate Isomerase for manipulation of glycolysis in yeast

Glucose-6-phosphate isomerase (Gis a naturally produced enzyme in most organisms that use glycolysis. It catalyzes the second step of the glycogenic cycle, which is the transformation of glucose-6-phosphate to fructose-6-phosphate. Overexpression of this enzyme in a vector with a strong constitutive promoter could lead to interesting metabolic effects and potentially increase the tolerance of the host organism to high concentrations of glucose.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 793
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 793
    Illegal BglII site found at 61
    Illegal BglII site found at 409
    Illegal BamHI site found at 702
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 793
    Illegal AgeI site found at 433
    Illegal AgeI site found at 1123
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 17


[edit]
Categories
Parameters
None