Project

Part:BBa_M36119

Designed by: Samson Phan, Noriko Ishisoko   Group: Stanford BIOE44 - S11   (2012-05-29)

GULO enzyme with start and stop

This construct includes the Kozak sequence, the gene sequence for the L-gulonolactone oxidase enzyme, and a stop codon. It should create a device that theoretically creates ascorbic acid in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1299
    Illegal PstI site found at 550
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1299
    Illegal PstI site found at 550
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1299
    Illegal BamHI site found at 275
    Illegal BamHI site found at 1278
    Illegal XhoI site found at 985
    Illegal XhoI site found at 1309
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1299
    Illegal PstI site found at 550
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1299
    Illegal PstI site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None