Coding

Part:BBa_M36106

Designed by: Andee Wallace, Taylor Nguyen, Chad Viergever   Group: Stanford BIOE44 - S11   (2011-05-03)

HY5 3 BS Promoter w/ Gemini, LacZ

Consists of promoter M36100, a ribosome binding site, the gene for gemini, and a transcriptional terminator. The promoter has three HY5 binding sites. When HY5 is present it may bind to the promoter, possibly preventing e. coli's DNA polymerase from binding, thus acting as a transcritional inhibitor. Depending on whether DNA polymerase can bind or not, transcription will ensue to produce gemini.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 926


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Categories
Parameters
None