Plasmid_Backbone

Part:BBa_M36092

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-05-08)


S. cerevisiae actuator test rig

S. cerevisae test vector for actuators. Has a Gal1 promoter which is galactose inducible and glucose repressible. Comes with high copy number origin, kanamycin resistance, and leucine yeast selection; can replace with low copy number origin if ordering from DNA 2.0.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4035
    Illegal XbaI site found at 4960
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4035
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4035
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4035
    Illegal XbaI site found at 4960
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4035
    Illegal XbaI site found at 4960
    Illegal AgeI site found at 1827
    Illegal AgeI site found at 3648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
//plasmidbackbone
Parameters
None