Coding

Part:BBa_M36080

Designed by: Dan Sakaguchi   Group: Stanford BIOE44 - S11   (2013-10-24)


Wild-type miraculin protein with His-tag at C-terminus

This is the coding gene for the protein naturally found in the miracle berry (Synsepalum dulcificum). The protein is his-tagged at the C-terminus (with a poly-glycine linker) for purification. In the berry the protein is produced as a glycoprotein dimer, in which form it has taste-modifying properties.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
    Illegal AgeI site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None