Coding

Part:BBa_M36060

Designed by: Taylor Marie Chavez   Group: Stanford BIOE44 - S11   (2015-10-23)


Poly-beta-hydroxybutyrate Growth Actuator

Part codes for the mRNA sequence for poly-beta-hydroxybutyrate polymerase, which synthesizes poly-3-hydroxybutyrate from glucose. Part should be inserted within pD431-SR with T7 promoter. Poly-3-hydroxybutyrate is a biodegradable polymer, which will break down after it fulfills its intended function and this part is intended to provide a means with which to synthesize the described useful polymer. Part is intended to be transformed into E. coli and grown on kanamycin plates. Expression of the vector can be induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None