Generator

Part:BBa_M36037

Designed by: Joe Schlicht, Trevor! Kalkus, and David Eduardo Arrambide Montemayor   Group: Stanford BIOE44 - S11   (2011-12-10)

Alpha Factor Mating Pheromone Producer

This gene encodes the thirteen amino acids that make up the Alpha factor yeast mating pheromone. It leads to apoptosis in MATa haploid yeast when unable to mate and if there are MATalpha haloid yeast it induces mating. It freezes diploid cells in the G1 growth phase. It is codon optimized for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 10
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None