Coding

Part:BBa_M36025

Designed by: Jon Rodriguez   Group: Stanford BIOE44 - S11   (2011-05-03)

phiX174 gene B - mutated rbs of K

Modified internal scaffolding protein from phiX174. Took the wt gene B from coliphage phiX174, and then mutated it synonymously in order to destroy the overlapping RBS of gene K

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 308
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 308
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 308
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 308
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None