Composite

Part:BBa_M36019

Designed by: Rachel Lim   Group: Stanford BIOE44 - S11   (2014-10-23)


Lactate Sensor

This sequence contains a promoter, RBS, coding sequence for lldR transcription factor, and a operon/promoter sequence. In the absence of lactate, lldR protein binds to the operon and prevents transcription downstream. Lactate binds to lldR and causes it to release the operon, so transcription downstream can continue.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 628
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None