Composite

Part:BBa_M36012

Designed by: Elliott Lui and Jesse Palmer   Group: Stanford BIOE44 - S11   (2011-04-28)

Glycogen Synthesis Actuator (ideal)

Input: PoPs Output: Glycogen overproduction

Expresses glgC mutant gene (coding for the glucose-1-phosphate adenylyltransferase), glgA gene (coding for glycogen synthase), and glgS gene (predicted glycogen synthesis protein). These genes are expressed normally for e. coli metabolism. This part is intended to be used for over expression and overproduction of glycogen.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1958
    Illegal PstI site found at 2390
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1958
    Illegal PstI site found at 2390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1958
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1958
    Illegal PstI site found at 2390
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1958
    Illegal PstI site found at 2390
    Illegal AgeI site found at 2582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2306


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
None