Part:BBa_M33356
L28H-fnr CDS
Long description: fnr (fumarate nitrate reductase) is a DNA-binding protein that is a global regulator involved in cellular respiration and metabolism during times of anaerobic cell growth (Melville et al 1996). As an iron-sulfur protein, however, fnr depends on its [4Fe-4S][+2] cluster to have functional ability in the cell. Upon exposure to oxygen, fnr undergoes a "rapid [4Fe-4S][+2] to [2Fe-2S][+2] cluster conversion...[and a] concomitant loss in site-specific DNA binding and dimerization" (Bates et al 2000), which results in fnr losing its acitvity in the cell in aerobic conditions.However, a substitution of Histidine on the 28th amino acid of fnr (which is Leucine in the WT [wildtype] fnr) results in a protein that is functional in aerobic growth conditions. The new mutant protein is called L28H-fnr in the literature. It is suggested that this added functionality of L28H-fnr occurs because the "His-28 substitution appears to stabilize the [4Fe-4S][+2] cluster of FNR-L28H in the presence of O2" (Bates et al 2000). The part's resistance to oxygen has allowed it to be used for more practical biosensors that can work in oxygen environments to "enable nitrate-dependent expression"(Li, Rabi, DeMoss 1985).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 777 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 778
Illegal PstI site found at 792
Illegal NotI site found at 7
Illegal NotI site found at 785 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 778 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 778
Illegal PstI site found at 792 - 1000COMPATIBLE WITH RFC[1000]
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