Regulatory

Part:BBa_M33351

Designed by: Sunny Kim   Group: Stanford BIOE44 - S010   (2010-06-06)

Ptod promoter activated by TodT protein

Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. The catabolic genes of the toluene dioxygenase pathway are transcribed from a single promoter termed Ptod, located upstream from the todX gene. The TodS/TodT TCS mediate Ptod in response to toluene and other aromatic hydrocarbons. Ptod is activated once the response regulator TodT is phosphorylated by the TodS sensor kinase in response to pathway substrates.

This part should be used in conjunction with BBa_M33350, the CDS for TodS downstream of an RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None