Coding

Part:BBa_M33334

Designed by: Christopher Brunson   Group: Stanford BIOE44 - S010   (2010-06-05)


CDS+RBS for Inorganic mercury-sensitive regulatory protein merR coding sequence with RBS

This contains the coding sequence for merR (mercury repressor protein) with an added medium strength RBS. It is part of the mer operon from Thermus thermophilus HB27, and binds to the mer promoter in the absence of inorganic mercury. if inorganic mercury is present, it binds to merR, which undergoes a conformational change that allows for transcription of the downstream operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None