Composite

Part:BBa_M333334

Designed by: biomagik@mit   Group: MIT 20.109 Fall07   (2008-05-06)


Produces Beta-carotene from the biochemical input GGPP

This part contains 3 genes, each of which codes for an enzyme in the carotenoid synthesis pathway: CrtB (Phytoene synthase), CrtI (Phytoene desaturase) and CrtY (Lycopene cyclase).

The conversion process is GGPP->Phytoene->Lycopene->B-carotene

Composite order: 1) Constitutive promoter BBa_J23100 2) CrtY 3) RBS BBa_B0034 4) CrtI 5) RBS BBa_J23100 6) CrtB 7) Terminator B0015


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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