Composite

Part:BBa_M31530

Designed by: Matt Gethers   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-28)


Refactored Portion of M13K07 Genome

I've refactored the segment of DNA between the unique HapI and BamHI sites. Following the guidlines in "Refactoring T7" by Chan et al, I've determined what functional components that I would like to be physically separated from one another (namely regulatory regions and ORFs)and set them apart in my design. I'm not sure that these modifications will yield a functional phage. Even viable phage result, I don't know what sort of emergent functions I will have created by refactoring the genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3251
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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