Coding

Part:BBa_M31520

Designed by: Han Zhu   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-27)


g8 modified to be independent of g3 promoter

This is a modified g8 that has point mutations to delete the promoter of g3 inside it. It also has an EcoRI restriction site added to the end. Therefore, it operates independent of g3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 229
    Illegal BglII site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 229
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None