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Part:BBa_M31500

Designed by: Tiffany Guo   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-27)


refactored M13K07 phage fragment

M13K07 fragment from bp 1-2222 (HpaI site to BamHI site), refactored 1) to eliminate overlaping regions between promoters, ribosome binding sites, open reading frames, and 2) to add restriction enzyme sites for easy separation of different parts of the genetic sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 225
    Illegal BamHI site found at 3399
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]


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